The methodological foundation of our research
// TECHNOLOGIES
These technologies enable the study and engineering of human bone and bone marrow tissues, providing cellular and molecular insights into the processes that shape their development, function, and disease.
// Technology 1
Human Stem Cell Line engineering
Engineered human cell lines provide standardized and unlimited cell sources, as well as facilitating genetic editing through CRISPR-Cas9. These include immortalized human umbilical vein endothelial cells (HUVECs) and bone marrow-derived mesenchymal stem cells (BM-MSCs), which retain robust expansion and multilineage differentiation potential. The resulting cellular tools support reproducible tissue formation, and enable cellular and molecular probing upon tissue development and function.
// Technology 2
Patient-Derived Mini-Bones (Humanized Ossicles)
Bioengineered bone and bone marrow organoids generated through developmental programs inspired by endochondral ossification. These patient-derived “mini-bones” mature in vivo and recreate key features of the patient's bone marrow niche, enabling translational studies of human hematopoiesis and disease.
// Technology 3
Advanced 3D Culture Systems
Custom-designed in vitro 3D culture platforms, including spheroids, organoids, 3D-printed bioreactors, and organ-on-chip technologies. These physiologically relevant models provide enhanced control and real-time monitoring of cellular interactions, more accurately recapitulating tissue architecture than conventional cell culture systems.
// Technology 4
Quantitative Microscopy
High-content imaging and quantitative image analysis to characterize cellular behavior, tissue organization, and dynamic biological processes across multiple spatial and temporal scales. Thick-section imaging combined with confocal microscopy enables high-resolution 3D reconstruction of complex tissues at cellular resolution.
// Technology 5
Multi-Omics Profiling
Integrated molecular profiling approaches, including single-cell transcriptomics, chromatin accessibility analysis (scATAC-seq), and spatial omics technologies, to resolve cellular heterogeneity, regulatory mechanisms, and tissue organization with high resolution.
