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2025
Compositional editing of extracellular matrices by CRISPR/Cas9 engineering of human mesenchymal cell lines
eLife
10.7554/eLife.96941.3
Prithiviraj S, Garcia Garcia A, Linderfalk K, Yiguang Bai, Ferveur S, Nilsen Falck L, Subramaniam A, Mohlin S, Hidalgo D, Dupard SJ, Bushan Raina D, Bourgine PE
CRISPR/Cas9 was exploited as a high precision system for editing the composition and function of engineered ECMs. RUNX2-edited eECMs exhibited impaired hypertrophy, reduced ectopic ossification and superior cartilage repair in a rat osteochondral defect.
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