New method article from Steven on the engineering of hematopoietic niches in bioreactors!
Steven Dupard describes in this Springer book chapter how 3D hematopoietic niches can be engineered in perfusion bioreactor. This is performed through the differentiation of human mesenchymal cells on specific scaffolding materials.
Congrats Dimitra for receiving a grant from Lund Innovation!
Dimitra Zacharaki received 300,000 SEK through the Exploratory Pre-Seed Programme of Lund Innovation to apply our hOssicle technology in modelling diseases.
New invited review article in press in Experimental Hematology
Congratulations to Paul Bourgine and all coauthors for their invited review article in press in Experimental Hematology!
Congrats Ani for receiving a grant from the Royal Physiographic Society of Lund!
Ani Grigoryan is awarded a 65,000 SEK grant from the Royal Physiographic Society of Lund which will be used to complete a part of her projects in the CTO lab.
Welcome to CTO Nasim!
Nasim Kalantari just joined our lab to conduct a 6-month degree project on the characterization of Ossicle-derived MSOD-B cells as part of her Master’s program on Molecular Medicine at Uppsala University. She has previously worked on human hematopoietic stem cells.
Andrei Chagin visits Lund University
We were honored to have professor Andrei Chagin from Karolinska Institute visit Lund to present his latest work on the epiphyseal stem cell niche. Very stimulating talk and discussion afterwards!
Day treat and retreat to nature for the CTO gang!
We explored the majestic automn palette of Skåne with a hike at Söderåsen national park and ended the day with some fine dining in Lund !
Congrats Paul for being awarded an ERC starting grant!
We are delighted to announce that Paul Bourgine has been awarded a prestigious 5-year ERC starting grant! Everyone at the CTO lab is excited about the upcoming studies on the hOssicle to model hematopoietic disorders.
Goodbye Melissa and Panajot!
This Friday we gathered to say goodbye to Melissa and Panajot who completed their master theses in our lab. Over the past 6 months they learned a lot and contributed to their individual projects with enthusiasm! We thank them and wish them the best of luck with their future plans!
Tommi Glorioso is a second year-student at the medical programme in Lund. As a summer scholarship project in the CTO lab, Tommi will be involved in the characterization of MSCs capable of cartilage formation in vitro and he will perform quantitative protein assays.
Our humanized ossicle on the cover of Trends in Mol. Med.!
We are very proud and excited to see our first review article published and our humanized ossicle featured on the cover of the current issue of Trends in Molecular Medicine!
The CTO lab warmly welcomes its new member Dimitra Zacharaki! Dimitra will perform a postdoctoral study that will focus on the application of our human ossicle model to study hematopoietic malignancies and solid tumor metastases.
First paper of the CTO lab!
The first review 'Development of Humanized Ossicles: Bridging the Hematopoietic Gap' of the CTO lab is online in the journal Trends in Molecular Medicine!
First edition of the mesenchymal stromal cell line retreat!
It was a great pleasure to welcome Ivan Martin, Andres Garcia-Garcia and Sébastien Pigeot in Sweden for this first edition of the mesenchymal stromal cell line retreat! Open discussion and synergy between our two groups were the key outcomes of this session.
Welcome to Panaj and Mel!
The CTO warmly welcomes Melissa Langlois and Panajot Kristofori! Two master students working on our in vitro and in vivo models of human hematopoiesis!
Welcome to Alejandro!
Alejandro will perform a postdoctoral study in the lab, centered around the production of cell-free extracellular matrices enriched with osteoinductive factors to enhance bone healing. Everything by ensuring the GMP to overcome clinical applications.
Congratulation Sonia for your graduation!
This is with a great pleasure that we announce the successful Master's thesis defense of Sonia! Congratulation for the good work, we wish you the very best for your brilliant future!
Our first CRISPR-edited cells!
Our first CRISPR-edited death-inducible mesenchymal line! Thanks Sonia for the precision of your molecular scissors!
Soran and Marvin's last days as master students
Today was the occasion for us to thank Soran and Marvin for their paramount contribution to the lab during the past 6 months, we wish you the best for your personal and professional future and we hope to see you in Sweden again!
Our first 3D Bioreactors are operating!
The lab just set up its first 3D perfusion-based bioreactors. This technology will open the gates to a broad range of functional investigations through in vitro engineering of tunable human hematopoietic stem cell niches.
Escape the cave & French cuisine!
As Indiana Jones, the team successfully uncovered the secrets and mysteries of Aztec treasures and gave their best impersonation of 20's style spy agents. After saving the world (twice), we gathered around a well-deserved French dinner.
Welcome Ani and Steven!
We are very happy to welcome Ani Grigoryan and Steven Dupard as lab members 7th and 8th! Ani will perform a postdoctoral study in the lab, centered around the exploitation of our human ossicle model. Instead, Steven just engaged in the Lund University PhD program. He will design customized hematopoietic niches in 3D in vitro systems. We wish them a happy start at the CTO lab!
Welcome aboard Sonia!
This week we welcome our new lab member, Sonia Ferveur! Sonia studies at the Ecole Superieure de Biotechnologie Strasbourg (ESBS, France) and will perform her master thesis for the next 6 months. Sonia aims at generating new MSOD lines using the Crispr/CaS9 system, in order to edit their transcriptional profiles. In that way, Sonia targets to tightly control what proteins a given MSOD line secrete or not. We wish her a successful research during her time here, as well as an exciting discovery of the Swedish life!
Our first Ossicles !
The lab just accomplished his first generation of ossicles! These consist in fully mature bone organs, with structure and composition identical to native bones. The process was achieved by priming our cells toward chondrogenesis for 3 weeks in vitro, followed by a 6 weeks in vivo incubation time (subcutaneous implantation in mice). The timing and efficiency of remodeling is simply remarkable, and lay the ground for investigating deeper how this endochondral mechanism function.
Teaching day @ the CTO!
The 25th of January, we hosted 12 PhD students & postdocs in the context of the Wallenberg research school in regenerative medicine. The course was centered around the methods for Skeletal regeneration, which we tried to explain via different practical workshops. We hope that the participants got a broad view of the key techniques in placed in our lab, and that they had fun in the mean time!
Merry christmas and a happy New Year!
During Christmas, most of the lab’s crew will return home to celebrate Christmas and the New Year. We wish you a merry Christmas and a happy New Year too. See you next year 🙂
The lab just received a brand new 3D printer, set up by Marvin. This new device will allow designing personalized pieces of equipment at affordable price. First prints are on the go!
Welcome to our newcomers!
Since November 3rd: Sujeethkumar Prithiviraj, Marvin Grischke and Soran Alatas joined the CTO lab!
Sujeeth has been hired as Research Assistant after obtaining his Master degree from Aachen university in Germany. He will exploit the ossicle model to gather information on the endochondral bone formation.
Marvin is part of the ETH Zürich and he is performing his Master Thesis here in Lund. He will aim at demonstrating that the properties of biological matrices can be finely tuned, similarly to the properties of their synthetic counterparts.
Soran is part of the EPFL in Lausanne and also came to the lab to prepare his Master Thesis. He is concentrating his efforts on quantifying the fate of hMSCs in engineered cartilage and bone tissues.