3D Engineering of Human Hematopoietic Niches in Perfusion Bioreactor
Springer Methods in Molecular Biology, 2021. Dupard S, Bourgine PE. Abstract The hematopoietic microenvironment, also referred to as hematopoietic niche, is a functional three-dimensional (3D) unit of the bone marrow (BM) that planar culture systems cannot recapitulate. Existing limitations of 2D protocols are
Engineering human hematopoietic environments through ossicle and bioreactor technologies exploitation
Experimental Hematology, 2020. Sommerkamp P, Mercier FE, Wilkinson AC, Bonnet D, Bourgine PE. Abstract The bone marrow microenvironment contains cellular niches that maintain the pool of hematopoietic stem and progenitor cells and support hematopoietic maturation. Malignant hematopoietic cells also co-opt normal cellular interactions in order to promote their
Extracellular matrix reinforced bioinks for 3D bioprinting human tissue
Advanced Materials, 2020. De Santis MM, Alsafadi HN, Tas S, Bölükbas DA, Prithiviraj S, Da Silva IAN, Mittendorfer M, Ota C, Stegmayr J, Daoud F, Königshoff M, Swärd K, Wood JA, Tassieri M, Bourgine PE, Lindstedt S, Mohlin S, Wagner DE. Abstract Recent
Orthotopic Bone Formation by Streamlined Engineering and Devitalization of Human Hypertrophic Cartilage
International Journal of Molecular Sciences, 2020. Pigeot S, Bourgine PE, Jaquiery C, Scotti C, Papadimitropoulos A, Todorov A, Epple C, Peretti GM, Martin I. Abstract Most bones of the human body form and heal through endochondral ossification, whereby hypertrophic cartilage (HyC) is formed and
Development of Humanized Ossicles: Bridging the Hematopoietic Gap
Trends in Molecular Medicine, 2020. Dupard SJ*, Grigoryan A*, Farhat S, Coutu DL, Bourgine PE. Abstract Ectopic 'humanized ossicles' (hOss) are miniaturized, engineered human bone organs in mice displaying a similar structure and function to native mouse bones. However, they are composed of human mesenchymal derived
Fate Distribution and Regulatory Role of Human Mesenchymal Stromal Cells in Engineered Hematopoietic Bone Organs
iScience, 2019. Bourgine PE, Fritsch K, Pigeot S, Takizawa H, Kunz L, Kokkaliaris KD, Coutu DL, Manz MG, Martin I, Schroeder T. Abstract The generation of humanized ectopic ossicles (hOss) in mice has been proposed as an advanced translational and fundamental model to study
Hard material modulation for (skeletal) tissue engineering purposes, Springer book series
Springer Reference book series: "Biomedical Engineering - Tissue Engineering and Regeneration" Bourgine PE. Abstract: This series Tissue Engineering consists of comprehensive reference texts encompassing the biological basis of tissue regeneration, basic principles of tissue engineering and the current state-of-the-art in tissue engineering of
In vitro biomimetic engineering of a human hematopoietic niche with functional properties
Proceedings of the National Academy of Science USA, 2018. Bourgine PE, Klein T, Paczulla AM, Shimizu T, Kunz L, Kokkaliaris KD, Coutu DL, Lengerke C, Skoda R, Schroeder T, Martin I. Abstact: In adults, human hematopoietic stem and progenitor cells (HSPCs) reside in
Engineering human bone marrow proxies
Cell Stem Cell, 2018. Bourgine PE, Martin I, Schroeder T. Abstract Recent advances in engineering complex organs in vitro inspire the development of human bone marrow equivalents to foster scientific discovery and innovative therapeutics. Here, we discuss challenges in generating relevant human bone marrow proxies, potential design principles, and future directions. Full article
Mouse and human HSPC immobilization in liquid culture by CD43/CD44-antibody coating
Blood, 2018. Loeffler D, Wang W, Hopf A, Hilsenbeck O, Bourgine PE, Rudolf F, Martin I, Schroeder T. Abstract: Keeping track of individual cell identifications is imperative to the study of dynamic single cell behavior over time. Highly motile hematopoietic stem and progenitor